The NucleoCassette is a highly specialised device, which holds the key to several of the unique features of the NucleoCounter YC-100. The fluorescent dye propidium iodide (PI) is immobilized in the interior flow channels of the disposable cassette. When the cassette has been loaded with approximately 50 µl of sample mixture the PI is dissolved and mixed with the sample, staining the nuclei of the yeast cells.
After placement in the NucleoCounter YC-100 the stained mixture is automatically transferred to the measurement chamber, where the fluorescent image is recorded. The measurement volume is as large as 1 µl.
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