Category : Bioscience, biotechnology, life health science (precision equipment, instruments, devices and accessories) > Cellular analysis systems, cells dispensing machine, cell culture monitoring, cell screening, cell imaging

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Guava Technologies Inc. (www.guavatechnologies.com) has introduced a new simple non-radioactive assay designed to improve the performance and sensitivity of cell proliferation assays compared to traditional population-based methods such as 3-Thymidine and MTT (or XTT).

The Guava CellGrowth assay, when used in conjunction with the Guava EasyCyte System, provides cell proliferation analysis at the single-cell level, offering cell proliferation assessments without the use of more complex fluorescence methods out to 5 generations. In addition, the assay offers a broader linear range and is more quantitative when compared to colorimetric methods.

The Guava CellGrowth Assay uses a well-characterised cell tracking dye that diffuses freely into cells and is retained within the cell without affecting cellular function. The dye is not transferred to adjacent cells. The Guava CellGrowth assay uses two dyes, a cell permanent painting dye and a cell impermanent DNA binding dye, to distinguish live or dead proliferated cells from live or dead resting cells. For each round of cell division, the relative fluorescence intensity of the dye is decreased by half. The assay is ideal for monitoring the growth of primary cultures, such as peripheral blood mononuclear cells, activated T or B cells, peripheral blood lymphocytes, thymocytes, hematopoietic cells, splenocytes and fibroblasts.

Used in conjunction with the Guava EasyCyte the Guava CellGrowth Assay provides researchers with a convenient 96-well format for multiple sample analyses increasing productivity and reducing intensive labour costs. The assay also eliminates the costs and safety issues around the use and disposal of toxic and radioactive chemicals.

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List of all articles in this sub-category Cellular analysis systems, cells dispensing machine, cell culture monitoring, cell screening, cell imaging

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