Enhancing single-cell analysis with accurate cell enumeration

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Guava Technologies, Inc. has introduced a new side scatter option for its Guava EasyCyte™ system that makes single-cell analysis with accurate cell enumeration more versatile and easier than ever before.

The side scatter feature allows researchers to analyse whole blood for different types of white blood cells, without having to stain or fix the cells. The granular structures in the cytoplasm of granulocytes, a type of white blood cell, cause more scattering of incident light than the more uniform cytoplasm of lymphocytes, another type of white blood cell. This makes it easier to perform a three-part differential on whole blood: assessing the percentage and number of granulocytes, monocytes, and lymphocytes in a given sample.

"The addition of side scatter will expand our opportunities in the immunology and oncology markets," said Larry Bruder, Guava vice president, worldwide marketing and business development. "Addition of side scatter functionality supports our goal to bring accessible and powerful, yet cost-effective cytometry to the life sciences market." Side scatter is also used for a variety of others applications within the life sciences market, such as a QC measure for cell line sample integrity, as a way to eliminate debris from your sample, or to discern infected from non-infected cells.

The Guava EasyCyte is a five-parameter system that is flexible, highly affordable, and ultra-compact. Since it requires only a few microlitres of sample volume, it saves precious and expensive cells, reagents, and compounds. The system is so easy to use, results are generated with less than a day's training. The Guava EasyCyte system runs each of Guava's turnkey assays, enabling easy, fast measurements of cell counting/viability, GFP expression and viability, apoptosis, cell cycle analysis, antigen detection, cell tracking, and more.

Guava Technologies, Inc., a privately held biotechnology and medical device company, is the leading provider of on-demand, easy-to-use single cell analysis systems. The Guava Personal Cell Analysis (PCA™) systems, including the Guava PCA, Guava PCA-96 and Guava EasyCyte systems, are integrated, fully optimised, micro-capillary cytometry systems with embedded absolute cell counting capability. Used worldwide by the life sciences, biotechnology, pharmaceutical industries, and clinical testing institutions (outside the United States and Europe), Guava's products have broad applications in scientific research and throughout the drug discovery and lead optimisation process, as well as for cell counting and optimisation of commercial bio-pharmaceutical production. Guava offers a variety of assays and dedicated software modules for the Guava PCA systems, enhancing the system's overall ease-of-use.

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