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Category : Optics (components, coatings, instruments and systems) > Optical instrumentation, systems and accessories

HSE/NPL Test slides used for measuring microscope performance

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The HSE/NPL Test Slide was developed by the Health & Safety Laboratory (HSL) on behalf of the Health & Safety Executive (HSE) in collaboration with PTR Optics and the National Physical Laboratory (NPL). Its purpose is to provide a reproducible phase object to check microscope performance prior to counting asbestos fibre samples. PTR Optics is the exclusive manufacturer of the HSE/NPL Test Slide.

The master engraving was produced by NPL using a ruling engine. It consists of 7 bands of 20 lines with widths ranging from 0.25µm to 1.1µm. The lines were produced by a V shaped inscriber with a depth to width ratio of 1:10. The bands are separated by 20mm gaps. The test zone is delineated by a rectangle bounded by deep grooves that can be viewed with a microscope at x100 magnification in either dark field or phase contrast mode. The table below gives the widths of the lines within each band and the associated pahse contrast induced when green light (λ = 530µm) passes through a line.

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