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Category : Bioscience, biotechnology, life health science (precision equipment, instruments, devices and accessories) > Cellular analysis systems, cells dispensing machine, cell culture monitoring, cell screening, cell imaging

Rapid computer-assisted absolute cell count and viability analysis

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Guava Technologies, Inc., a biomedical products company focused on the life science R&D and clinical diagnostics markets, today announced that it has launched Guava ViaCount® EasyTMFit. EasyFit is an innovative cluster analysis algorithm providing more precise, accurate and automated absolute cell counting and viability measurements.

Accuracy, reproducibility, and standardisation are essential to successful outcomes in laboratories that perform cell count and viability assessments. Traditional methods for counting cells and assessing cell viability are time consuming. Moreover such methods often produce variable results since they rely on inherently subjective visual discrimination of dye-stained cells using a hemacytometer and microscope.

Guava Technologies’ ViaCount Assay is a simple, accurate, and reproducible test for absolute cell counting and viability measurements that overcomes many of the limitations of traditional methods. The ViaCount Assay reduces statistical variation by sampling large numbers of single cells, and minimises operator variability by using fluorescence intensity measurements to categorize cells as live, dead, or apoptotic. This simple and robust Guava ViaCount assay has now been enhanced with the new Guava Easy™Fit software feature that automates and standardises cell counting and viability assessments.

“EasyFit provides users of our product with a means to operate the system without the need for manual adjustment of gating parameters,” stated Keith Olson, director of product commercialisation at Guava Technologies. “Guava ViaCount remains one of the most popular applications used on our platforms, and the enhancements introduced by this product are a direct response to feedback from our customers.”

This new Guava ViaCount software provides a powerful computer-assisted clustering algorithm to group cellular distributions based on their positions in a 3D matrix. Cells are classified and counted based on three individual measurements of each cell, including the fluorescence intensity values for the live and dead cell based on uptake of the dyes in Guava’s ViaCount solution.

About Guava Technologies

Guava Technologies, Inc. is a leading provider of integrated, fully optimised microcytometry and cell counting systems. Guava serves the worldwide life science, biotechnology and pharmaceutical industries, offering a state-of-the-art, comprehensive portfolio of products designed to accelerate discovery and increase productivity with accessible, easy-to-use solutions to the biologist’s testing problems available right at the lab bench. Guava’s products have broad applications in scientific research, drug discovery, cell counting, and optimisation of commercial bioproduction. Guava’s technology also offers potential for use in clinical testing and blood processing, as well as other areas where single cell counting is important.

The Guava PCA systems offer an integrated, optimised approach that takes the guesswork out of single cell analysis and counting. Based on patented micro-capillary technology, the Guava PCA systems are available in three formats, the Guava PCA, the Guava PCA-96, and the Guava PCA-96 AFP. These cell analysis systems are flexible, highly affordable and ultra-compact, requiring only a few microlitres of sample volume, thus saving precious and expensive cells, reagents and compounds. Guava offers a variety of assays and dedicated software modules for the Guava PCA systems, enhancing the system’s overall ease-of-use.

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