Category : Bioscience, biotechnology, life health science (precision equipment, instruments, devices and accessories) > Microarray products

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Available from Genetix (www.genetix.com) are a range of microarray products suitable for screening the efficacy of short-interfering RNA (siRNA) molecules in RNA Interference (RNAi) studies.

At the forthcoming RNAi Europe meeting in London (18-19th October 2004), Genetix will be demonstrating its QArrayMini microarrayer and aQuire microarray scanner with blue laser option that provides a perfect combination for carrying out RNAi optimisation experiments.

RNAi has been developed as a powerful molecular biology tool to achieve targeted gene silencing in cells. Double-stranded siRNA molecules (typically 20-25 nucleotides in length) can be introduced into mammalian cells by a variety of techniques (see Genetix Application Note: RNAi and Microarrays). Once inside the cell, these molecules interact with complementary target mRNA and specific proteins to form an RNA-induced silencing complex (RISC), which reduces or ablates the level of protein expression from the gene of interest.

It is usually necessary to test and optimise a variety of oligonucleotide sequences for their efficacy in silencing a given gene, which can be done in a parallel and high-throughput manner by microarraying. The synthetic siRNA duplexes or the plasmids which are eventually processed into siRNA molecules in the cell can be arrayed with a matrix of lipid transfection reagents onto a microarray slide using the QArrayMini or QArray2. The slide is then overcoated with a monolayer of adherent cells which subsequently become transfected upon contact with the arrayed spots. In the case of an RNAi screen where the cell line is expressing a GFP fusion protein to the target gene, the efficacy of each siRNA molecule is assessed by the formation of plaques (non-fluorescent regions) in the lawn of fluorescent GFP-expressing cells. Images of GFP fusion protein expressing cells can be acquired and analysed using the Genetix aQuire scanner with optional blue laser.

For further information on the use of microarrays for RNAi optimisation studies please contact Genetix.

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